Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Stereotype Content Model02:16

Stereotype Content Model

15.5K
The Stereotype Content Model (SCM) was first proposed by Susan Fiske and her colleagues (Fiske, Cuddy, Glick & Xu, 2002; see also Fiske, 2012 and Fiske, 2017). The SCM specifies that when someone encounters a new group, they will stereotype them based on two metrics: warmth—or that group’s perceived intent, and how likely they are to provide help or inflict harm—and competence—or their ability to carry out that objective. Depending on the warmth-competence...
15.5K
Measurement of Air Content in Concrete01:23

Measurement of Air Content in Concrete

663
Air content measurement in concrete is critical for ensuring structural integrity and durability of concrete structures, especially in environments prone to severe weather conditions. Accurate air content analysis optimizes concrete's resistance to freeze-thaw cycles and enhances its workability and strength. Several methods are standardized under ASTM guidelines to measure the air content in fresh concrete, each suitable for different concrete types and conditions.
The pressure method,...
663
Distribution of Cytoplasmic Content02:33

Distribution of Cytoplasmic Content

4.7K
Cytokinesis segregates a cell’s chromosomes and organelles into its daughter cells. Organelles divide and grow prior to cell division but cannot be synthesized de novo; therefore, cells must receive at least one copy of each organelle to survive. Currently, many of the details of how the organelles are distributed are not yet fully elucidated.
Distribution of cytoplasmic determinants
The cytoplasm contains various organelles, as well as salts, proteins, and water. The distribution of...
4.7K
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

12.5K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
12.5K
Moisture Content and Bulking of Aggregate01:10

Moisture Content and Bulking of Aggregate

474
The moisture content of aggregates is a crucial factor in construction, particularly in concrete mixing, as it influences the total water required in the mix. Moisture content represents the water coated on the exterior surface of the aggregate existing in a saturated and surface-dry condition. The total water content of a moist aggregate is the sum of its moisture content and water absorption.
When aggregates are exposed to rain or sit in stockpiles, they absorb moisture, which must be...
474
Genetic Screens02:46

Genetic Screens

5.8K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of <i>in vitro</i> blood-brain barrier models in central nervous system new drug development: methodological challenges and translational perspectives.

Expert opinion on drug delivery·2026
Same author

Lumbar-ventricular cerebrospinal fluid pressure discrepancy in leptomeningeal metastasis associated intracranial hypertension.

Fluids and barriers of the CNS·2026
Same author

Long-acting IL-7 induces distinct transcriptomic features in peripheral T cells of patients with solid tumors.

JCI insight·2026
Same author

Loss of ABCA3 disrupts lipid balance and leads to AMPK-dependent suppression of SREBP1 in glioblastoma stem cells.

Oncogene·2026
Same author

Improved tumor control with adjuvant radiotherapy in patients with central neurocytoma: a multicenter study.

Journal of neuro-oncology·2026
Same author

Separation of large droplets from an oil-in-water emulsion using a deterministic lateral displacement (DLD) microfluidic chip.

Scientific reports·2026

Related Experiment Video

Updated: Feb 9, 2026

Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening
14:22

Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening

Published on: September 18, 2016

9.0K

3D Cell-Based High-Content Screening (HCS) Using a Micropillar and Microwell Chip Platform.

Sang-Yun Lee1,2,3, Il Doh4, Do-Hyun Nam1,2,5

  • 1Department of Health Sciences and Technology, Samsung Advanced Institute for Health Sciences and Technology (SAIHST) , Sungkyunkwan University , Seoul , 06351 , Republic of Korea.

Analytical Chemistry
|June 12, 2018
PubMed
Summary

This study introduces a 3D cell culture chip for high-content screening, enabling detailed analysis of drug effects. The platform successfully identified phospho-EGFR (p-EGFR) inhibition by drugs in 3D cancer models.

More Related Videos

A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
15:04

A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity

Published on: May 5, 2009

25.8K
High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents HPHC
11:38

High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents HPHC

Published on: May 10, 2016

12.8K

Related Experiment Videos

Last Updated: Feb 9, 2026

Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening
14:22

Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening

Published on: September 18, 2016

9.0K
A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
15:04

A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity

Published on: May 5, 2009

25.8K
High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents HPHC
11:38

High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents HPHC

Published on: May 10, 2016

12.8K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Drug Discovery

Background:

  • 3D cell culture offers more physiologically relevant models than 2D cultures.
  • Micropillar/microwell chips enable high-throughput 3D cell culture.
  • Previous applications were limited to cell viability, hindering deeper analysis.

Purpose of the Study:

  • To develop a 3D cell-based high-content screening (HCS) platform using micropillar/microwell chips.
  • To optimize antibody staining for 3D cultured cells within the chip.
  • To assess drug-induced changes in protein expression (p-EGFR) and cellular structures.

Main Methods:

  • Utilized a micropillar/microwell chip for 3D cell culture.
  • Developed and optimized antibody staining protocols for immobilized 3D cells.
  • Performed HCS to analyze phospho-EGFR (p-EGFR), F-actin, and nuclei.
  • Validated p-EGFR inhibition using Western blot.

Main Results:

  • Successfully stained and analyzed p-EGFR, F-actin, and nuclei in 3D cultured cells.
  • Identified p-EGFR inhibition by 72 different drugs.
  • Correlated chip-based p-EGFR expression with F-actin and nuclear staining.
  • Western blot confirmed the chip data for p-EGFR inhibition.

Conclusions:

  • The developed platform enables robust HCS of 3D cell cultures.
  • This approach provides valuable phenotypic and biological information for drug action studies.
  • The platform is effective for identifying drug-induced modulation of specific protein targets like p-EGFR in 3D cancer models.