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Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

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Long non-coding RNA expression profile in Cdk5-knockdown mouse skin.

Kaiyuan Ji¹, Ruiwen Fan¹, Junzhen Zhang¹

¹College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China.

|June 12, 2018

Summary

This summary is machine-generated.

This study reveals a novel long non-coding RNA, TCONS_00049140, that regulates skin thickening by down-regulating Krt80. This finding offers insights into the molecular mechanism of Cyclin-dependent kinase 5 (Cdk5) function in skin.

Keywords:

Cdk5-knockdown Krt80 Melanogenesis lncRNA-mRNA interaction

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Last Updated: Feb 9, 2026

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

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Published on: November 28, 2018

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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

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Published on: April 10, 2018

Area of Science:

Molecular Biology
Dermatology
Genetics

Background:

The molecular mechanisms regulating skin homeostasis and Cdk5 function remain incompletely understood.
Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cellular processes, including skin biology.

Purpose of the Study:

To elucidate the role of lncRNAs in Cdk5-mediated skin regulation.
To identify specific lncRNAs involved in skin thickening and pigmentation.

Main Methods:

Generated Cdk5-knockdown mice for lncRNA sequencing.
Utilized bioinformatics analyses (miRBase, KEGG PATHWAY, RNAplex) to identify lncRNA-mRNA interactions.
Performed luciferase assays and overexpression studies in mouse melanocytes.

Main Results:

Identified 4533 novel lncRNAs, with 693 significantly differentially expressed in Cdk5-knockdown skin.
Discovered potential lncRNA regulators of pigmentation and identified 97 lncRNA-mRNA interactions.
Confirmed TCONS_00049140 binds to Krt80, down-regulates its expression, and promotes melanocyte proliferation and melanin production, leading to skin thickening.

Conclusions:

TCONS_00049140 is a key lncRNA regulating skin thickening via Krt80.
This study provides a novel direction for understanding Cdk5's molecular mechanism in skin biology.