Related Experiment Video
Updated: Feb 9, 2026

Analytical Techniques for Assaying Nitric Oxide Bioactivity
Published on: June 18, 2012
Nitric oxide inhibitory constituents from Siegesbeckia pubescens
Hari Jang1, Jin Woo Lee1, Jun Gu Kim1
1College of Pharmacy, Chungbuk National University, Cheongju 28160, Republic of Korea.
Abstract:
Two new sesquiterpenoids (1 and 2) and a new ent-pimarane type diterpenoid (3), together with eighteen known compounds (4-21), were isolated from the whole plants of Siegesbeckia pubescens. The structures of the new compounds were determined on the basis of 1D-, 2D NMR and HRESIMS data. All compounds were evaluated for their inhibitory effects on LPS-induced nitric oxide production in RAW 264.7 macrophages. Of these, highly oxygenated germacrane type sesquiterpenoids (1-2 and 13-14) showed significant inhibitory effects with IC50 values ranging from 3.9 to 16.8 μM.
More Related Videos
Related Concept Videos
Nitric Oxide Signaling Pathway
Oxidation Numbers
Pyruvate Oxidation
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
Oxidation-Reduction Reactions
Excitatory and Inhibitory Effects of Neurotransmitters
Oxidation of Alcohols
The process of oxidation in a chemical reaction is observed in any of the three forms:

