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Updated: Feb 9, 2026

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
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Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

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Cross-linked cytochrome P450 BM3 aggregates promoted by Ru(II)-diimine complexes bearing aldehyde groups

Minh Quan Do1, Evelynn Henry1, Mallory Kato1

  • 1San José State University, Department of Chemistry, One Washington Square, San José, CA 95192-0101, United States.

Journal of Inorganic Biochemistry
|June 12, 2018
PubMed
Summary

Novel cross-linking agents improve immobilized enzyme stability and activity. Cross-linked enzyme aggregates (CLEAs) of cytochrome P450 BM3 variants show high activity recovery and enhanced stability after reduction, boosting overall protein performance.

Keywords:
Cross-linked enzyme aggregatesHeterogeneous biocatalysisP450 peroxygenaseRu(II)-diimine complexes with aldehyde

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