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Amplicon Sequencing using the Long-Read Sequencing Technologies
Published on: August 29, 2025
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Current and Future Molecular Testing in NSCLC, What Can We Expect from New Sequencing Technologies?
Simon Garinet1,2, Pierre Laurent-Puig3,4, Hélène Blons5,6
1INSERM UMR-S1147, Paris Sorbonne Cite University, 75270 Paris Cedex 06, France. simon.garinet@aphp.fr.
Journal of Clinical Medicine
|June 13, 2018
Summary
Deeper molecular testing in lung cancer aids targeted drug use. Comprehensive profiling identifies new predictive markers for personalized treatment strategies.
Area of Science:
- Oncology
- Genomics
- Personalized Medicine
Background:
- Lung cancer treatment has evolved with new first-line therapies and advanced molecular diagnostics.
- The integration of high-throughput molecular technologies necessitates evaluating their role in optimizing targeted drug selection.
Purpose of the Study:
- To review recent advancements in personalized lung cancer medicine.
- To assess the clinical utility of comprehensive molecular profiling and large-scale sequencing strategies.
- To discuss emerging biomarkers and challenges in identifying new predictive markers.
Main Methods:
- Review of recent publications (2012-2018) on lung cancer treatment and molecular profiling.
- Analysis of therapeutic predictive biomarkers, including EGFR and ALK inhibitors, anti-BRAF/MEK combinations, and novel fusions (NRG1, NTRKs).
- Discussion of immunotherapy and technical aspects of comprehensive molecular profiling.
Main Results:
- Second- and third-generation EGFR and ALK inhibitors are effective, with insights into secondary resistance mechanisms.
- Emerging biomarkers like NRG1 and NTRK fusions show therapeutic potential.
- Large-scale sequencing may enhance the predictive value of individual biomarkers.
Conclusions:
- Comprehensive molecular profiling is crucial for guiding personalized lung cancer treatment.
- Addressing technical challenges in molecular testing is key to identifying new predictive markers.
- Advanced molecular strategies offer promising avenues for improving patient outcomes in lung cancer.
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