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Amplicon Sequencing using the Long-Read Sequencing Technologies
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Current and Future Molecular Testing in NSCLC, What Can We Expect from New Sequencing Technologies?

Simon Garinet1,2, Pierre Laurent-Puig3,4, Hélène Blons5,6

  • 1INSERM UMR-S1147, Paris Sorbonne Cite University, 75270 Paris Cedex 06, France. simon.garinet@aphp.fr.

Journal of Clinical Medicine
|June 13, 2018
PubMed

Insights

Deeper molecular testing in lung cancer aids targeted drug use. Comprehensive profiling identifies new predictive markers for personalized treatment strategies.

Area of Science:

  • Oncology
  • Genomics
  • Personalized Medicine

Background:

  • Lung cancer treatment has evolved with new first-line therapies and advanced molecular diagnostics.
  • The integration of high-throughput molecular technologies necessitates evaluating their role in optimizing targeted drug selection.

Purpose of the Study:

  • To review recent advancements in personalized lung cancer medicine.
  • To assess the clinical utility of comprehensive molecular profiling and large-scale sequencing strategies.
  • To discuss emerging biomarkers and challenges in identifying new predictive markers.

Main Methods:

  • Review of recent publications (2012-2018) on lung cancer treatment and molecular profiling.
  • Analysis of therapeutic predictive biomarkers, including EGFR and ALK inhibitors, anti-BRAF/MEK combinations, and novel fusions (NRG1, NTRKs).
  • Discussion of immunotherapy and technical aspects of comprehensive molecular profiling.

Main Results:

  • Second- and third-generation EGFR and ALK inhibitors are effective, with insights into secondary resistance mechanisms.
  • Emerging biomarkers like NRG1 and NTRK fusions show therapeutic potential.
  • Large-scale sequencing may enhance the predictive value of individual biomarkers.

Conclusions:

  • Comprehensive molecular profiling is crucial for guiding personalized lung cancer treatment.
  • Addressing technical challenges in molecular testing is key to identifying new predictive markers.
  • Advanced molecular strategies offer promising avenues for improving patient outcomes in lung cancer.
Keywords:
NGSlung cancermolecular analysisoncogene drivers

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