Related Experiment Video
Updated: Feb 9, 2026
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
Published on: January 17, 2015
Basics of Antibody Phage Display Technology
Line Ledsgaard1, Mogens Kilstrup2, Aneesh Karatt-Vellatt3
1Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby DK-2800, Denmark. Lineledsgaard@hotmail.com.
Phage display technology is a powerful tool for antibody discovery, particularly in antivenom research. This review details its principles and applications for identifying antibodies against animal toxins.
Area of Science:
- Biotechnology
- Immunology
- Toxinology
Background:
- Antibody discovery is crucial in modern medicine.
- Phage display technology is gaining traction in toxinology and antivenom research.
Purpose of the Study:
- To review the principles and applications of phage display technology.
- To focus on antibody discovery targeting animal toxins.
Main Methods:
- Explanation of the M13 phage lifecycle.
- Overview of phage display technology basics.
- Discussion of antigen display methods and library types (naïve vs. immunized).
Main Results:
- Highlights factors influencing phage display success rates.
- Presents examples from antivenom research.
Conclusions:
- Phage display offers a robust method for discovering antibodies against animal toxins.
- This review provides comprehensive insights into the technology for antivenom development.
Related Concept Videos
02:27Energy Basics
01:21Basicity of Aliphatic Amines
To measure the basicity of amines, two conventions are generally used. The first defines Kb as the basicity constant for the deprotonation reaction of water by the amine, as presented in Figure 1. Conventionally, lower Kb indicates higher...
01:22Basic Operations on Signals
Time Reversal mirrors a continuous-time signal about the vertical axis at t=0. This is achieved by substituting t with −t. For example, if a signal x(t) is considered, the time-reversed signal is x(−t). This operation can be graphically represented, showing the mirrored signal.
02:52VSEPR Theory and the Basic Shapes
01:28Basic Concept
Length, which measures the distance traveled by an object, is a fundamental concept in engineering mechanics. We use coordinates relative to a reference point to describe the distance. Length not only helps to describe the...
01:10Antibody Structure
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...