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[MicroRNA-218 promotes osteosarcoma cell apoptosis by down-regulating oncogene B lymphoma mouse Moloney leukemia

Gui-Hua Lai1, Ai-Lan Huang, Zhi Zhao

  • 1Department of Human Anatomy, First Affiliated Hospital of Bengbu Medical College, Bengbu 233030, China.E-mail: lailgh198272@ foxmail.com.

Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University
|June 13, 2018
PubMed
Abstract

Insights

MicroRNA-218 (miR-218) acts as a tumor suppressor in osteosarcoma (OS) by promoting cancer cell apoptosis. This effect is achieved by down-regulating the oncogene BMI-1 (B lymphoma mouse Moloney leukemia virus insertion region 1).

Area of Science:

  • Molecular biology
  • Oncology
  • Gene regulation

Background:

  • Osteosarcoma (OS) is a primary bone malignancy with a complex genetic landscape.
  • MicroRNAs (miRNAs) are key regulators of gene expression and have been implicated in various cancers, including OS.
  • The role of microRNA-218 (miR-218) in OS pathogenesis remains to be fully elucidated.

Purpose of the Study:

  • To investigate the tumor-suppressive function of miR-218 in osteosarcoma.
  • To explore the underlying molecular mechanisms by which miR-218 exerts its effects in OS cells.
  • To identify potential targets of miR-218 in osteosarcoma.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) was used to assess miR-218 expression in OS tissues and cell lines.
  • Transfection with miR-218 mimics and anti-miR-218 mimics was performed in Saos-2 OS cells.
  • Cell viability, apoptosis (using CCK-8 assay, annexin V-FITC staining, Western blotting for cleaved PARP), and target gene expression (luciferase assay, qRT-PCR, Western blotting for BMI-1) were analyzed.

Main Results:

  • miR-218 expression was significantly downregulated in OS tissues compared to adjacent tissues.
  • Overexpression of miR-218 reduced OS cell viability and induced apoptosis, evidenced by increased cleaved PARP and flow cytometry.
  • BMI-1 (B lymphoma mouse Moloney leukemia virus insertion region 1) was identified as a direct target of miR-218, with its expression reduced at both mRNA and protein levels upon miR-218 upregulation.

Conclusions:

  • miR-218 functions as a tumor suppressor in osteosarcoma.
  • miR-218 promotes OS cell apoptosis.
  • The tumor-suppressive role of miR-218 is mediated through the downregulation of its target gene, BMI-1.

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