Related Experiment Video

Updated: Feb 9, 2026

A GPC3-targeting Bispecific Antibody, GPC3-S-Fab, with Potent Cytotoxicity
11:13

A GPC3-targeting Bispecific Antibody, GPC3-S-Fab, with Potent Cytotoxicity

Published on: July 12, 2018

9.2K

Anti-PSMA/CD3 Bispecific Antibody Delivery and Antitumor Activity Using a Polymeric Depot Formulation

Wilhem Leconet1, He Liu2, Ming Guo2

  • 1Department of Urology, Weill Cornell Medical College-New York Presbyterian Hospital, New York, New York. wil2011@med.cornell.edu.

Molecular Cancer Therapeutics
|June 13, 2018
PubMed

Insights

A novel injectable polymer system successfully delivered BiJ591, a bispecific T-cell engager, for sustained prostate cancer treatment. This controlled release enhanced therapeutic efficacy compared to daily injections.

Area of Science:

  • Oncology
  • Biomaterials Science
  • Drug Delivery Systems

Background:

  • Small therapeutic proteins offer promise for cancer treatment but face challenges due to short plasma half-life and limited stability.
  • Controlled long-term delivery of hydrophilic and potentially unstable biologicals remains a significant hurdle in clinical applications.

Purpose of the Study:

  • To develop and evaluate an in situ forming, injectable polymeric depot system for the controlled delivery of BiJ591, a bispecific T-cell engager (BiTE).
  • To assess the stability, release kinetics, and therapeutic efficacy of BiJ591 delivered via the polymeric system in prostate cancer models.

Main Methods:

  • Utilized diblock (DB) and triblock (TB) biodegradable polyethylene glycol-poly(lactic acid) (PEG-PLA) copolymers solubilized in tripropionin to create an injectable depot.
  • Administered the BiJ591-loaded polymeric formulation subcutaneously in mice with prostate cancer xenografts.
  • Evaluated BiJ591 release profiles, stability, T-cell activation, and tumor growth inhibition.

Main Results:

  • The triblock copolymer formulation (TB1/DB4) demonstrated sustained release of BiJ591 for up to 21 days after a single subcutaneous injection.
  • The BiJ591-TB1/DB4 formulation showed significant therapeutic activity in both low and high PSMA-expressing prostate cancer xenografts.
  • This controlled delivery approach was more effective than daily intravenous administration of BiJ591.

Conclusions:

  • Injectable PEG-PLA copolymer depots provide a viable strategy for the controlled, long-term delivery of small therapeutic proteins like BiJ591.
  • This formulation enhances the therapeutic efficacy of BiTEs in prostate cancer treatment, offering a promising alternative to conventional administration methods.

More Related Videos

Evaluation of the In vivo Antitumor Activity of Polyanhydride IL-1α Nanoparticles09:57

Evaluation of the In vivo Antitumor Activity of Polyanhydride IL-1α Nanoparticles

Published on: June 28, 2021

2.4K
Tracking Bispecific Antibody-Induced T Cell Trafficking Using Luciferase-Transduced Human T Cells10:19

Tracking Bispecific Antibody-Induced T Cell Trafficking Using Luciferase-Transduced Human T Cells

Published on: May 12, 2023

1.7K

Related Concept Videos

Insulin Formulations: Types and Delivery01:27

Insulin Formulations: Types and Delivery

Insulin preparations are categorized by their duration of action into short-acting and long-acting types. Two strategies are used to modify insulin's absorption and pharmacokinetic profile: slowing the absorption post-subcutaneous injection, or altering human insulin's amino acid sequence or protein structure. These changes retain the insulin's ability to bind to the insulin receptor, but alter its behavior in solution or after injection.
Short-acting insulins are divided into...
765
Olefin Metathesis Polymerization: Ring-Opening Metathesis Polymerization (ROMP)01:16

Olefin Metathesis Polymerization: Ring-Opening Metathesis Polymerization (ROMP)

Ring-opening metathesis polymerization or ROMP involves strained cycloalkenes as starting materials. The mechanism of ROMP proceeds by reacting cycloalkene with Grubbs catalyst to give metallacyclobutane intermediate which undergoes a ring-opening reaction to form new carbene. The new carbene reacts with another molecule of cycloalkene. Repetition of these steps leads to the formation of an unsaturated open-chain polymer product. All these steps are reversible, however, relieving the ring...
3.2K
Actin Polymerization01:42

Actin Polymerization

Actin polymerization occurs through the head-to-tail association of binding sites on monomeric actin or G-actin to form filamentous or F-actin. The polymerization can be divided into three phases ̶  nucleation, elongation, and steady-state phase.
The nucleation phase involves forming a stable nucleus consisting of three actin monomers to form a new actin filament. Actin-binding proteins such as formins and Arp2/3 complex help filament growth post-nucleation. The Formins form straight...
8.6K
Antibody Structure01:10

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
65.7K
Cartesian Form for Vector Formulation01:26

Cartesian Form for Vector Formulation

The Cartesian form for vector formulation is a process to calculate  the moment of force using the position and force vectors. The moment of force is defined as the cross-product of these vectors, making it a vector quantity. The Cartesian form of the position and force vectors involves unit vectors, which can be used to express the cross-product in determinant form.
1.1K
Resultant Moment: Vector Formulation01:30

Resultant Moment: Vector Formulation

When a force is applied to an object, the tendency of the object to rotate about a point is known as its moment. If multiple forces are acting on an object, the sum of moments of all the forces acting on a body can be expressed as the resultant moment of the system. The resultant moment can be considered a vector quantity that can be added and subtracted like any other vector.
The resultant moment of a system of forces can be calculated through vector formulation. For example, if we consider...
3.9K