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Herpes simplex virus type 1 and neuronal cells--a special cell-virus interaction
Intervirology
|January 1, 1985
Summary
Researchers isolated unique
Area of Science:
- Virology
- Cell Biology
- Neuro-oncology
Background:
- Rat brain glioma cells exhibit semipermissive herpes simplex virus (HSV) replication.
- HSV replication in glioma cells is dependent on the multiplicity of infection.
Purpose of the Study:
- To isolate and characterize glioma cells that survive HSV infection.
- To investigate the unique interaction between glioma cells and HSV.
Main Methods:
- Infection of rat brain glioma cells with HSV at low multiplicity.
- Isolation of surviving glioma cells without special treatments.
- Assessment of viral replication, superinfection resistance, and interferon induction in survivor cells.
Main Results:
- 'Survivor' glioma cells were successfully isolated, unlike BHK or 3T3 cells.
- Survivor cells ceased infectious virus production after two subcultures.
- Survivor cells demonstrated high resistance to superinfection and produced HSV-specific antigens and DNA.
Conclusions:
- A unique glioma cell-HSV interaction allows for the isolation of resistant survivor cells.
- Survivor cells exhibit distinct characteristics, including resistance to HSV superinfection.
- These findings offer insights into cellular resistance mechanisms against viral infections in brain tumors.