Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

1.8K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.8K
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

9.0K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
9.0K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.5K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.5K
Mass Spectrometry: Isotope Effect01:13

Mass Spectrometry: Isotope Effect

4.3K
Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
4.3K
Mass Spectrometry of Amines01:15

Mass Spectrometry of Amines

5.4K
In mass spectroscopy, amines undergo fragmentation to give parent ions with odd molecule weights. This observed mass spectrum follows the nitrogen rule; a molecule with an odd number of nitrogen atoms produces a molecular ion with an odd molecular weight. Amines undergo fragmentation through α cleavage, producing nitrogen-containing cations—iminium ions—and alkyl radicals. Mass spectra of aromatic and cyclic aliphatic amines exhibit strong molecular ion peaks, but acyclic...
5.4K
Chemical Ionization (CI) Mass Spectrometry01:21

Chemical Ionization (CI) Mass Spectrometry

1.6K
The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
1.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Serine Octamer Substitution Reactions With α-Hydroxy Acids.

Rapid communications in mass spectrometry : RCM·2026
Same author

Molecular Framework Modification in Mass Spectrometry: Atom Exchange, Insertion, and Deletion.

Journal of the American Society for Mass Spectrometry·2026
Same author

Early-stage drug discovery in a new-generation ultrahigh-throughput mass spectrometry platform.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

High-Throughput Reactive Desorption Electrospray Ionization Mass Spectrometry for Targeted Derivatization and Analysis of Poorly Ionized Small Molecules.

Analytical chemistry·2026
Same author

High-Throughput Small-Scale Platform for Synthesis, Characterization, and Modeling of Per- and Polyfluoroalkyl Substances Analogs.

Environmental science & technology letters·2026
Same author

Accelerated Synthesis of Pyrazoles Mediated by Water Microdroplets.

ACS sustainable chemistry & engineering·2026

Related Experiment Video

Updated: Feb 9, 2026

Synthesis and Mass Spectrometry Analysis of Oligo-peptoids
11:44

Synthesis and Mass Spectrometry Analysis of Oligo-peptoids

Published on: February 21, 2018

11.4K

Mass Spectrometry for Synthesis and Analysis.

R Graham Cooks1, Xin Yan1,2

  • 1Department of Chemistry, Purdue University, West Lafayette, Indiana 47906, USA;

Annual Review of Analytical Chemistry (Palo Alto, Calif.)
|June 13, 2018
PubMed
Summary

Mass spectrometry, the science of ions, enables accelerated reactions, synthesis, and material transformation. Research highlights ion/surface collisions, tissue imaging, and diagnostics, with a focus on chirality and the origins of life.

Keywords:
accelerated reactionsambient ionizationcancer diagnosticsion chemistryion soft landingmolecular imagingorigin of lifereaction monitoring

More Related Videos

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
09:09

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

Published on: October 13, 2020

5.1K
Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
08:56

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: March 8, 2020

7.9K

Related Experiment Videos

Last Updated: Feb 9, 2026

Synthesis and Mass Spectrometry Analysis of Oligo-peptoids
11:44

Synthesis and Mass Spectrometry Analysis of Oligo-peptoids

Published on: February 21, 2018

11.4K
Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
09:09

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

Published on: October 13, 2020

5.1K
Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
08:56

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: March 8, 2020

7.9K

Area of Science:

  • Analytical Chemistry
  • Physical Chemistry
  • Materials Science

Background:

  • Mass spectrometry is a fundamental technique for studying ions.
  • Instrumentation is crucial for analytical applications in mass spectrometry.
  • Purdue University's Aston Laboratories conducts research in ion-based science.

Purpose of the Study:

  • To describe research in mass spectrometry focusing on ion reactions, synthesis, and applications.
  • To highlight advancements in ion/surface collision processes.
  • To showcase applications in tissue imaging and diagnostics, including chirality and origins of life.

Main Methods:

  • Generating and measuring ion properties.
  • Studying accelerated ion reactions in solution and small-scale synthesis.
  • Investigating ion/surface collision processes like surface-induced dissociation (SID) and ion soft landing.
  • Applying mass spectrometry to tissue imaging and diagnostics.

Main Results:

  • Demonstrated accelerated ion reactions and synthesis.
  • Characterized ion/surface collision dynamics, including SID and ion soft landing.
  • Showcased mass spectrometry applications in tissue imaging and diagnostics.
  • Explored chirality and origins of life chemistry.

Conclusions:

  • Mass spectrometry is a versatile tool for diverse scientific investigations.
  • Advancements in instrumentation drive analytical applications.
  • The research contributes to understanding ion behavior and developing new diagnostic tools.