Related Experiment Video

Updated: Feb 9, 2026

Modeling Spontaneous Metastatic Renal Cell Carcinoma mRCC in Mice Following Nephrectomy
11:27

Modeling Spontaneous Metastatic Renal Cell Carcinoma mRCC in Mice Following Nephrectomy

Published on: April 29, 2014

17.2K

Long non-coding RNA GAS5 sensitizes renal cell carcinoma to sorafenib via miR-21/SOX5 pathway

Lei Liu1, Xinlu Pang1, Wenjin Shang1

  • 1a Department of kidney transplantation , the First Affiliated Hospital of Zhengzhou University , Zhengzhou , Henan Province , P. R. China.

Cell Cycle (Georgetown, Tex.)
|June 14, 2018
PubMed

Insights

A long noncoding RNA, GAS5, is downregulated in sorafenib-resistant renal cell carcinoma (RCC). Restoring GAS5 may overcome resistance, suggesting GAS5 as a potential therapeutic target and biomarker for RCC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Sorafenib improves survival in renal cell carcinoma (RCC) but resistance occurs.
  • Understanding sorafenib resistance mechanisms and identifying biomarkers are crucial for effective RCC treatment.

Purpose of the Study:

  • To investigate the role of long noncoding RNA GAS5 in sorafenib resistance in RCC.
  • To identify GAS5 as a potential biomarker and therapeutic target for sorafenib treatment in RCC.

Main Methods:

  • Quantitative analysis of GAS5 expression in sorafenib-responsive and nonresponsive RCC.
  • In vitro and in vivo experiments involving GAS5 overexpression and knockdown in RCC cells.
  • Investigation of the molecular mechanism involving GAS5, miR-21, and SOX5.

Main Results:

  • GAS5 was significantly downregulated in sorafenib-nonresponsive RCC.
  • GAS5 overexpression sensitized nonresponsive RCC cells to sorafenib.
  • GAS5 knockdown induced sorafenib resistance in responsive RCC cells.
  • GAS5 acts as a competing endogenous RNA, repressing miR-21 and subsequently SOX5.

Conclusions:

  • GAS5 downregulation is associated with poor response to sorafenib in RCC.
  • The GAS5/miR-21/SOX5 axis plays a critical role in regulating sorafenib resistance in RCC.
  • GAS5 represents a potential predictive biomarker and therapeutic target for sorafenib treatment in RCC.
Keywords:
GAS5RCCSorafenib

More Related Videos

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

26.3K
Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood09:40

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Published on: November 28, 2018

7.8K

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.0K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.7K
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.7K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
14.9K
Nursing Code of Ethics01:29

Nursing Code of Ethics

The Nursing Code of Ethics sets the ethical benchmark for the profession, and guides nurses in ethical analysis and decision making at the societal, organizational, and clinical levels. The code encompasses showing compassion and respect for the patient, their families, and communities in all circumstances while committing to providing patient-centered care. In addition, the code states that nurses must advocate for the patient by defending a cause or recommendation to protect their rights,...
4.6K
C4 Pathway and CAM01:27

C4 Pathway and CAM

Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
49.3K