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Pubescenosides E⁻K, Seven New Triterpenoid Saponins from the Roots of Ilex pubescens and Their Anti-Inflammatory
Xiaoxu Qiao1, Mengying Ji2, Yunda Yao3
1Joint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education of the People's Republic of China, International Institute for Translational Chinese Medicine, University of Chinese Medicine, Guangzhou 510006, China. qiaoxiaoxu87@126.com.
Abstract:
Seven new triterpenoid saponins (1⁻7), together with three known ones (8⁻10), were isolated from Ilex pubescens. Elucidation of their structures was performed based on high-resolution electrospray ionisation mass spectrometry (HR-ESI-MS), infrared spectra (IR), and nuclear magnetic resonance (NMR) spectroscopic data. The anti-inflammatory activity of the isolates toward lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages was investigated. The results demonstrated that compounds 3, 5, and 6 inhibited the expression of inducible nitric oxide synthase (iNOS) protein in comparison with LPS stimulation in RAW264.7 cells.
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