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Updated: Feb 9, 2026

Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation
Published on: March 12, 2015
Graftable SCoMPIs enable the labeling and X-ray fluorescence imaging of proteins
Sarah Hostachy1, Marie Masuda2, Takayuki Miki2
1Laboratoire des Biomolécules, LBM , Département de Chimie , École Normale Supérieure , PSL University , Sorbonne Université , CNRS , 75005 Paris , France .
Abstract:
Bio-imaging techniques alternative to fluorescence microscopy are gaining increasing interest as complementary tools to visualize and analyze biological systems. Among them, X-ray fluorescence microspectroscopy provides information on the local content and distribution of heavy elements (Z ≥ 14) in cells or biological samples. In this context, similar tools to those developed for fluorescence microscopy are desired, including chemical probes or tags. In this work, we study rhenium complexes as a convenient and sensitive probe for X-ray fluorescence microspectroscopy. We demonstrate their ability to label and sense exogenously incubated or endogenous proteins inside cells.
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