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Updated: Feb 9, 2026

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue
Published on: April 27, 2012
Visualization of Gene Expression Patterns by In Situ Hybridization on Early Stages of Development of Xenopus laevis
Heithem M El-Hodiri1, Lisa E Kelly2
1Center for Molecular and Human Genetics, Nationwide Children's Hospital Research Institute, Columbus, OH, USA. Heithem.El-Hodiri@NationwideChildrens.org.
Abstract:
In situ hybridization performed using whole fixed embryos provides accurate and detailed visualization of gene expression patterns. These patterns are useful for investigating spatial patterns of gene expression in normally developing embryos but can also be useful in investigating the effects of genetic or environmental changes on expression of genetic markers characteristic of particular tissues, organs, or genetic pathways. Our lab's protocol for whole-mount in situ hybridization is presented.
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