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Updated: Feb 9, 2026

An Integrated Approach for Microprotein Identification and Sequence Analysis
Published on: July 12, 2022
[Identification and pedigree analysis of ABO subgroup B303]
Fawen Chen1, Haihua Xie, Xiaojun Yang
1Clinical College of Fujian Provincial Hospital, Fujian Medical University, Fuzhou, Fujian 350001, China. chenfawen@163.com.
Objective:
To explore the molecular mechanism of a case of ABO discrepancies based on the results of blood group serology.
Methods:
Five cases of the two-generation pedigrees were analyzed. ABO genotypes were determined using serological tests. DNA sequence analysis was performed on exon 6, exon 7 and intron 3 of the 5 cases to confirm the genotypes of a proband with B subgroup and 4 family members.
Results:
There were 3 cases of subgroup AB3 and 1 case of subgroup B3 among the 5 family members. The genotypes were identified as A102/B303 and O02/B303, respectively. B303 differed from B101 by intron 3 point mutation (intron3 + 5G>A).
Conclusion:
The point mutation of intron 3 (intron 3+5G>A) is specific in B303.
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