GlycoStore: a database of retention properties for glycan analysis

Area of Science:

• Glycomics
• Biochemistry
• Analytical Chemistry

Background:

• Glycan analysis is crucial for understanding glycoproteins, glycolipids, and biotherapeutics.
• Existing databases like GlycoBase have limitations in data scope and modeling.
• Publicly available experimental datasets are fragmented and require curation.

Purpose of the Study:

• To develop and present GlycoStore, a comprehensive database for glycan structure and retention data.
• To enhance features and address limitations of previous glycan resources.
• To support and underpin current glycan analysis workflows.

Main Methods:

• Curated collection of chromatographic, electrophoretic, and mass-spectrometry data.
• Integration of public datasets from GlycoBase and new data from BTI, Macquarie University, and Ludger Ltd.
• Data acquisition using hydrophilic interaction chromatography (HILIC) U/HPLC, reversed-phase (RP)-U/HPLC, porous graphitized carbon (PGC) chromatography with ESI-MS/MS, and capillary electrophoresis with laser-induced fluorescence (CE-LIF).

Main Results:

• GlycoStore provides access to approximately 850 unique glycan structures.
• Over 8500 retention positions are documented across various analytical methods.
• The database integrates data from multiple sources, enhancing previous resources.

Conclusions:

• GlycoStore is a valuable, regularly updated resource for detailed glycan analysis.
• It consolidates diverse analytical data, improving accessibility and utility for researchers.
• The database supports current workflows in glycomics and biopharmaceutical analysis.