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Multiple sequence-specific DNA binding activities are eluted from chicken nuclei at low ionic strengths.
Nucleic Acids Research
|June 11, 1985
Summary
Researchers identified DNA-binding proteins in chicken cells that interact with specific gene regions. These proteins bind to key sites in the chicken beta A-globin gene and also to human beta-globin and other gene sequences.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Nuclear proteins play crucial roles in gene regulation.
- Identifying sequence-specific DNA-binding proteins is essential for understanding gene control.
Purpose of the Study:
- To investigate DNA sequence-specific binding proteins from chicken nuclei.
- To characterize the binding sites of these proteins on globin and other gene sequences.
Main Methods:
- Filter binding assays
- DNA footprinting analyses
- Fractionation of nuclear proteins
Main Results:
- Chicken erythrocyte nuclear proteins bind to two sites in the 5' flanking region of the chicken beta A-globin gene.
- These proteins also bind to a site 5' to the human beta-globin gene, near hypersensitive regions.
- Binding sites include the CACCC consensus sequence in the chicken beta A-globin gene.
- Sequence-specific binding was also observed for human N-ras and c-myc gene sequences.
Conclusions:
- Specific DNA-binding proteins from chicken nuclei recognize regulatory elements in globin genes.
- These findings contribute to understanding the molecular mechanisms of gene regulation across species.