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A Predicted Mannoprotein Participates in Cryptococcus gattii Capsular Structure.

Julia Catarina Vieira Reuwsaat1, Heryk Motta1, Ane Wichine Acosta Garcia1

  • 1Laboratório de Fungos de Importância Médica e Biotecnológica, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.

Msphere
|June 14, 2018
PubMed
Summary

Cryptococcus gattii mannoprotein Krp1 is vital for capsule structure and cell wall integrity, influencing glucan distribution. However, Krp1 is not essential for cryptococcosis virulence in vivo.

Keywords:
Cryptococcus gattiicapsular polysaccharidemannoprotein

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Area of Science:

  • Mycology
  • Immunology
  • Cell Biology

Background:

  • Cryptococcus gattii is a yeast-like pathogen causing cryptococcosis.
  • The polysaccharide capsule is a major virulence factor, composed of GXM, GalXM, and mannoproteins.
  • The role of mannoproteins in capsule assembly and pathogenicity is poorly understood.

Purpose of the Study:

  • To characterize the function of a predicted mannoprotein, Krp1, in Cryptococcus gattii.
  • To investigate Krp1's role in capsule assembly, cell wall structure, and pathogenicity.

Main Methods:

  • Generation of Krp1 loss-of-function and gain-of-function mutants.
  • Evaluation of capsular architecture and cell wall phenotypes.
  • Assessment of GXM release, cell-associated polysaccharide thickness, and macrophage phagocytosis.
  • Murine model of cryptococcosis and antigenicity testing of recombinant Krp1.

Main Results:

  • Krp1 null mutants showed increased sensitivity to cell wall stressors and higher GXM release.
  • Loss of Krp1 affected capsule thickness and early macrophage phagocytosis.
  • Krp1 is antigenic and recognized by cryptococcosis patient serum.
  • No difference in virulence was observed in a murine model.

Conclusions:

  • Krp1 is crucial for Cryptococcus gattii cell wall structure and capsule assembly.
  • Krp1 modulates glucan distribution in the cell wall, impacting capsule integrity.
  • Krp1 is not essential for in vivo virulence but plays a role in host-pathogen interactions.