Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Orbiviruses from Culicoides in Florida.

E C Greiner, T L Barber, J E Pearson

    Progress in Clinical and Biological Research
    |January 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Time Refraction of Spin Waves.

    Physical review letters·2021
    Same author

    Large Spin-Wave Bullet in a Ferrimagnetic Insulator Driven by the Spin Hall Effect.

    Physical review letters·2016
    Same author

    Realization of a spin-wave multiplexer.

    Nature communications·2014
    Same author

    Universal method for separating spin pumping from spin rectification voltage of ferromagnetic resonance.

    Physical review letters·2013
    Same author

    Unanticipated proximity behavior in ferromagnet-superconductor heterostructures with controlled magnetic noncollinearity.

    Physical review letters·2013
    Same author

    Thermoelectric detection of spin waves.

    Physical review letters·2013

    Bluetongue virus (BTV) and Epizootic hemorrhagic disease virus (EHDV) were detected in Florida biting midges. Culicoides insignis was identified as a potential new vector for BTV, impacting cattle health.

    Area of Science:

    • Veterinary Entomology
    • Arbovirology
    • Molecular Epidemiology

    Background:

    • Biting midges (Culicoides spp.) are vectors for economically significant arboviruses affecting livestock.
    • Understanding Culicoides species' role in virus transmission is crucial for disease control in cattle.
    • Previous studies have primarily focused on Culicoides variipennis as the main vector for bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) in the New World.

    Purpose of the Study:

    • To investigate the presence of orbiviruses, including BTV and EHDV, in Culicoides biting midges collected in Florida.
    • To identify potential new Culicoides vectors for BTV and EHDV.
    • To assess the epidemiological significance of Culicoides insignis in the transmission of BTV.

    Main Methods:

    Related Experiment Videos

  • Collection of 45,484 Culicoides spp. specimens from cattle farms in Florida between 1980 and 1983.
  • Attempted virus isolation using cell cultures and intravenous inoculation of embryonated chicken eggs.
  • Serotyping of isolated viruses.

    • Main Results:

    • Bluetongue virus (BTV) serotype 2 was isolated from Culicoides insignis, marking the first recovery from this species and the second New World Culicoides species implicated in BTV transmission.
    • Epizootic hemorrhagic disease virus (EHDV) serotype 2 was recovered from Culicoides variipennis.
    • No viruses were isolated from Culicoides arboricola, Culicoides stellifer, and Culicoides niger.

    Conclusions:

    • Culicoides insignis, a neotropical midge found in Florida, the Caribbean, and South America, is confirmed as a BTV vector.
    • The potential role of C. insignis in BTV transmission could explain antibody distribution in regions lacking C. variipennis.
    • These findings highlight the importance of identifying and monitoring various Culicoides species for effective arbovirus control in livestock populations.