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Automated High-Throughput Flow Cytometry for High-Content Screening in Antibody Development.

Yana Wang1, Tomoki Yoshihara2, Samson King1

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|June 15, 2018
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Summary
This summary is machine-generated.

High-throughput flow cytometry (HTFC) streamlines antibody discovery by enabling multiplexed cell line analysis. This robotic workflow enhances screening efficiency and accelerates the identification of therapeutic antibody leads.

Keywords:
HTFCantibody screeninghigh-throughput flow cytometrymultiplexon-cell binding

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Area of Science:

  • Biotechnology
  • Drug Discovery
  • Immunology

Background:

  • Flow cytometry is sensitive and accurate but limited by tedious sample preparation for high-throughput screening.
  • Antibodies are increasingly important as therapeutic agents, necessitating faster discovery methods.
  • Current methods struggle to meet the demand for supporting antibody discovery programs.

Purpose of the Study:

  • To develop a seamless high-throughput flow cytometry (HTFC) sample preparation and readout workflow.
  • To enhance the efficiency and throughput of antibody discovery programs.
  • To enable multiplexed analysis for simultaneous assessment of multiple parameters.

Main Methods:

  • Utilized the HighRes modular robotic system and IntelliCyt iQue Screener PLUS for workflow automation.
  • Developed a method for multiplexing multiple cell lines within a single well.
  • Integrated measurement of on-target activity, nonspecific activity, and antibody concentration.

Main Results:

  • Achieved a seamless HTFC sample preparation and readout workflow.
  • Enabled simultaneous quantitation of multiple parameters including on-target and nonspecific activity, and antibody concentration.
  • Demonstrated increased productivity and accelerated antibody lead discovery.

Conclusions:

  • The developed HTFC workflow overcomes sample preparation bottlenecks, significantly increasing throughput.
  • Multiplexing capabilities allow for comprehensive analysis in a single assay, improving efficiency.
  • This automated approach accelerates the discovery and development of therapeutic antibodies.