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Automated High-Throughput Flow Cytometry for High-Content Screening in Antibody Development
Yana Wang1, Tomoki Yoshihara2, Samson King1
11 Lead Discovery, Oncology Drug Discovery Unit, Takeda Pharmaceuticals, Cambridge, MA, USA.
High-throughput flow cytometry (HTFC) streamlines antibody discovery by enabling multiplexed cell line analysis. This robotic workflow enhances screening efficiency and accelerates the identification of therapeutic antibody leads.
Area of Science:
- Biotechnology
- Drug Discovery
- Immunology
Background:
- Flow cytometry is sensitive and accurate but limited by tedious sample preparation for high-throughput screening.
- Antibodies are increasingly important as therapeutic agents, necessitating faster discovery methods.
- Current methods struggle to meet the demand for supporting antibody discovery programs.
Purpose of the Study:
- To develop a seamless high-throughput flow cytometry (HTFC) sample preparation and readout workflow.
- To enhance the efficiency and throughput of antibody discovery programs.
- To enable multiplexed analysis for simultaneous assessment of multiple parameters.
Main Methods:
- Utilized the HighRes modular robotic system and IntelliCyt iQue Screener PLUS for workflow automation.
- Developed a method for multiplexing multiple cell lines within a single well.
- Integrated measurement of on-target activity, nonspecific activity, and antibody concentration.
Main Results:
- Achieved a seamless HTFC sample preparation and readout workflow.
- Enabled simultaneous quantitation of multiple parameters including on-target and nonspecific activity, and antibody concentration.
- Demonstrated increased productivity and accelerated antibody lead discovery.
Conclusions:
- The developed HTFC workflow overcomes sample preparation bottlenecks, significantly increasing throughput.
- Multiplexing capabilities allow for comprehensive analysis in a single assay, improving efficiency.
- This automated approach accelerates the discovery and development of therapeutic antibodies.
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