Related Experiment Video
Updated: Feb 9, 2026

Mouse Genome Engineering Using Designer Nucleases
Published on: April 2, 2014
Generation of artificial sequence-specific nucleases via a preassembled inert-template
Xianjin Xiao1, Tongbo Wu1, Feidan Gu1
1Beijing National Laboratory for Molecular Sciences and MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering , College of Chemistry and Molecular Engineering , Peking University , Beijing 100871 , China .
Abstract:
Sequence specific nucleases are important tools for processing nucleic acids in a predictable way. Herein, we demonstrate a conceptually new approach for generating sequence-specific nucleases via a preassembled inert-template (PAIT). A fairly stable DNase I/inert-DNA complex was prepared with a customized sequence specificity for a target DNA which contains a sequence complementary to the inert-DNA template. The complex could efficiently cleave the targeted sequence within either a long double-stranded DNA or a single-stranded DNA without affecting other unrelated DNA strands. The discrimination factor against single-base mismatch strands within a 14 nt target region was as high as 25.3. The strategy was also successfully applied to RNase A. Our findings may hold great potential for the development of a number of new powerful enzymatic tools.
More Related Videos
11:04RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
Published on: May 19, 2019
09:52Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq
Published on: April 19, 2013
Related Concept Videos
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
DNA as a Genetic Template
DNA as a Genetic Template
Cis-regulatory Sequences
Natural and Artificial Concepts
Sequences