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Author Spotlight: MAPP Protocol – Advancing Glycan Analysis

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Published on: September 29, 2023

Carbohydrate microarrays for screening functional glycans.

Jaeyoung Pai¹, Ji Young Hyun¹, Jieun Jeong¹

¹Center for Biofunctional Molecules , Department of Chemistry , Yonsei University , Seoul 03722 , Korea .

Chemical Science

|June 15, 2018

Summary

Carbohydrate microarrays can now screen for glycans that trigger cellular responses like reactive oxygen species (ROS) production. This method identifies functional glycans by detecting ROS, offering a new tool for biological research.

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Published on: February 14, 2019

Area of Science:

Glycobiology
Cellular immunology
Biotechnology

Background:

Carbohydrate microarrays are established for analyzing glycan binding.
Their application in studying glycan-mediated cellular responses remains limited.

Purpose of the Study:

To develop a carbohydrate microarray approach for screening biologically active glycans.
To identify glycans that stimulate reactive oxygen species (ROS) production via cell-surface lectin binding.

Main Methods:

Utilized a carbohydrate microarray assay combined with a fluorescent ROS probe.
Quantified ROS levels in cells interacting with immobilized glycans.

Main Results:

Identified specific functional glycans that enhance ROS production upon binding to cell-surface lectins.
Observed decreased ROS levels when using ROS scavengers or NADPH oxidase inhibitors, confirming the pathway.

Conclusions:

Glycan microarrays are effective for simultaneously screening multiple glycans.
This approach can identify glycans that elicit cellular responses, such as ROS production, through lectin interactions.