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Volatilization01:10

Volatilization

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Volatilization gravimetry is an analytical technique that measures the mass lost due to the volatilization of the substance. This technique is used to estimate the amount of volatile material in a sample. To perform this method, heat a known amount of the sample to a high temperature in a crucible or other suitable vessel. The volatile substance in the sample evaporates, and the vapor is completely expelled from the crucible either by heating the sample or bubbling a stream of inert gas through...
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Molecules and Compounds02:38

Molecules and Compounds

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Atoms and Molecules
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Organic Compounds03:02

Organic Compounds

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All living things are formed mostly of carbon compounds called organic compounds. The category of organic compounds includes both natural and synthetic compounds that contain carbon. Although a single, precise definition has yet to be identified by the chemistry community, most agree that a defining trait of organic molecules is the presence of carbon as the principal element, bonded to hydrogen and other carbon atoms. However, some carbon-containing compounds such as carbonates, cyanides, and...
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Elements and Compounds01:27

Elements and Compounds

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Pure substances consist of only one type of matter. A pure substance can be an element or a compound. An element consists of only one type of atom, while a compound consists of two or more types of atoms held together by a chemical bond.
Elements
Elements are classified as atomic or molecular based on the nature of their basic units. They are unique forms of matter with specific chemical and physical properties that cannot break down into smaller substances by ordinary chemical reactions. There...
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Directly Acting Muscle Relaxants: Dantrolene and Botulinum Toxin01:26

Directly Acting Muscle Relaxants: Dantrolene and Botulinum Toxin

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Directly acting muscle relaxants like dantrolene and botulinum toxin (BoNT) have distinct mechanisms and applications. Dantrolene, a hydantoin derivative, acts on the ryanodine receptor (RYR1) in skeletal muscle cells. RYR1 are calcium channels present at the sarcoplasmic reticulum membrane. In response to excitation, they release calcium ions from the sarcoplasmic reticulum to the cytosol. Calcium promotes actin-myosin-mediated contraction of muscles.
The binding of dantrolene to the RYR1...
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Solubility of Ionic Compounds02:55

Solubility of Ionic Compounds

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Solubility is the measure of the maximum amount of solute that can be dissolved in a given quantity of solvent at a given temperature and pressure. Solubility is usually measured in molarity (M) or moles per liter (mol/L). A compound is termed soluble if it dissolves in water.
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Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
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Data on volatile compounds produced by serotype D Clostridium botulinum.

Satoshi Nojima1, Takao Myoda1, Kazuki Toeda1

  • 1Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri Hokkaido 099-2493, Japan.

Data in Brief
|June 15, 2018
PubMed
Summary

This study identified volatile compounds produced by Clostridium botulinum D-CB16 using gas chromatography/mass spectrometry. Twenty-one novel compounds were detected, indicating their production by this specific bacterial strain.

Keywords:
BotulismClostridium botulinumDVB/CAR/PDMS, divinylbenzene/carboxen/polydimethylsiloxaneEI, Electron impactFood poisoningGC, gas chromatographyGas chromatography/mass spectrometryMS, mass spectrometrySPME, Solid-phase microextractionTYG, trypticase peptone/yeast extract/glucoseVolatile compounds

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Area of Science:

  • Microbiology
  • Analytical Chemistry
  • Food Safety

Background:

  • Clostridium botulinum is a bacterium known for producing potent toxins.
  • Understanding the metabolic byproducts of C. botulinum is crucial for detection and safety.
  • Serotype D C. botulinum (D-CB16) is a specific strain requiring detailed analysis.

Purpose of the Study:

  • To identify and characterize the volatile compounds produced by serotype D Clostridium botulinum (D-CB16).
  • To differentiate between volatile compounds present in the growth medium and those produced by bacterial metabolism.
  • To establish a baseline for volatile compound production by D-CB16 in a defined medium.

Main Methods:

  • Cultivation of Clostridium botulinum D-CB16 in trypticase peptone/yeast extract/glucose (TYG) medium.
  • Solid-phase microextraction (SPME) for capturing volatile compounds.
  • Gas chromatography/mass spectrometry (GC/MS) for separation and identification of volatile compounds.

Main Results:

  • Thirty-five volatile compounds were identified in the uninoculated TYG medium.
  • Thirty-four volatile compounds were identified in the TYG medium after D-CB16 cultivation.
  • Twenty-one volatile compounds were uniquely detected in the medium containing D-CB16, indicating bacterial origin.

Conclusions:

  • Clostridium botulinum D-CB16 produces a distinct profile of volatile compounds.
  • GC/MS analysis coupled with SPME is effective for identifying bacterial volatile metabolites.
  • These findings contribute to the understanding of C. botulinum metabolism and potential detection markers.