Related Experiment Video
Updated: Feb 9, 2026

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
Published on: January 30, 2017
mRNA microarray data of FACS purified bovine small and large luteal cells
Vijay S Baddela1, Arpna Sharma1, Dirk Koczan2
1Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany.
Abstract:
Transcriptome profiling of FACS purified small luteal cells (SLC) and large luteal cells (LLC), isolated from mid-cycle corpora lutea (day 10-11), was performed using Affymetrix GeneChip Bovine Gene 1.0 ST Arrays. Gene expression was recorded using an Affymetrix gene chip scanner 3000. The corresponding expression array intensity (.CEL) files were deposited in the Gene Expression Omnibus (GSE106641). The subsequent expression analyses of CEL files were carried out for identifying important transcripts and their functions associated with SLC and LLC. These data have been comprehensively evaluated and interpreted in the companion article, "Global gene expression analysis indicates that small luteal cells are involved in extracellular matrix modulation and immune cell recruitment in the bovine corpus luteum" [1].
Related Concept Videos
Regulated mRNA Transport
Nuclear Export of mRNA
pre-mRNA Processing
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
mRNA Stability and Gene Expression
Cis-acting Elements involved in mRNA stability
Nonsense-mediated mRNA Decay
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Chromatin Structure Regulates pre-mRNA Processing
The chromatin structure, especially...

