Identification of five new variable restriction sites in HSV-1 DNA

Restriction endonuclease analysis of herpes simplex virus type 1 (HSV-1) DNA has shown that individual strains differ from one another. The differences can be recognized by the presence or absence of cleavage sites due to base changes, small insertions and occasional deletions. In a study in which 13 HSV-1 isolates from South Africa were analysed, a total of 5 new variable restriction sites were identified using the restriction endonucleases Hind III, Eco RI and Bgl II.