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Micro-Ribonucleic Acid Profiles From Microarray in Ankylosing Spondylitis.

Hye-Mi Jin1, Young-Nan Cho1, Seung-Jung Kee2

  • 1Department of Rheumatology, Research Institute of Medical Sciences, Chonnam National University Medical School and Hospital, Gwangju, South Korea.

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MicroRNA (miRNA) profiling in ankylosing spondylitis (AS) revealed distinct expression patterns. Specifically, hsa-miR-424-5p was elevated while hsa-miR-377 was decreased in synovial fluid cells of AS patients.

Keywords:
Ankylosing spondylitismicro-ribonucleic acidmicroarray analysis

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Area of Science:

  • Molecular Biology
  • Genetics
  • Immunology

Background:

  • Ankylosing spondylitis (AS) is a chronic inflammatory disease affecting the axial skeleton.
  • MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and are implicated in various diseases, including autoimmune disorders.

Purpose of the Study:

  • To identify candidate micro-ribonucleic acids (miRNAs) dysregulated in peripheral blood and synovial fluid mononuclear cells of patients with ankylosing spondylitis (AS).
  • To investigate the potential role of specific miRNAs in the pathogenesis of AS.

Main Methods:

  • MicroRNA microarray analysis was performed on samples from three AS patients and three healthy controls.
  • Quality control of total RNA was assessed using Agilent Bioanalyzer 2100.
  • Hierarchical clustering was used to evaluate storage condition impacts on miRNA expression.
  • MiScript primer assays validated miRNA expression levels.

Main Results:

  • Microarray screening identified 887 miRNAs.
  • In AS peripheral blood mononuclear cells, five miRNAs were downregulated and hsa-miR-424-5p was upregulated.
  • In AS synovial fluid mononuclear cells, 16 miRNAs were downregulated and hsa-miR-424-5p was upregulated.
  • Validation confirmed increased hsa-miR-424-5p and decreased hsa-miR-377 in AS synovial fluid cells.

Conclusions:

  • hsa-miR-424-5p is upregulated and hsa-miR-377 is downregulated in synovial fluid mononuclear cells of AS patients.
  • These miRNAs may play functional roles in AS pathogenesis through distinct molecular pathways.