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Published on: August 25, 2023
Protective Effect of Tetrandrine in a Rabbit Model of Osteoarthritis
Fengfeng Wu1, Juntao Xu2, Zaihua Zhu3
1Department of Orthopaedic Surgery, Huzhou Central Hospital, Huzhou, China.
Objectives:
This study aims to investigate the antiinflammatory effect of tetrandrine in a rabbit model of osteoarthritis.
Patients And Methods:
The cruciate ligaments and medial menisci of 36 female New Zealand white rabbits (each weighing 2.0-3.0 kg) were resected. Six weeks after surgery, the animals were randomly divided into three groups (n=12 in each): tetrandrine (20 mg/kg/day), indomethacin (3 mg/kg/day) and control (saline 10 mL/day) groups. After 14 days of treatment, the results were assessed by macroscopic observation, histological evaluation, measurement of the levels of interleukin-1 beta, tumor necrosis factor-alpha and nitric oxide in the synovial fluid and messenger ribonucleic acid expression of metalloproteinase-3 in synovia.
Results:
In the tetrandrine group, the pathological changes were more attenuated than in the control group. Levels of interleukin-1 beta, tumor necrosis factor-alpha, nitric oxide and metalloproteinase-3 in the synovial fluid or synovium were all significantly suppressed in the tetrandrine group compared with the control group. There were no significant differences between the tetrandrine and indomethacin groups.
Conclusion:
The results of this study demonstrate that tetrandrine may protect against the development of experimentally induced osteoarthritis.
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