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Updated: Feb 9, 2026

Fluorescence detection methods for microfluidic droplet platforms
Published on: December 10, 2011
Highly sensitive and selective determination of Hg(II) based on microfluidic chip with on-line fluorescent
Guilong Peng1, Yi Chen2, Ruoyu Deng2
1Key Laboratory of Eco-Environment of Three Gorges Region of Ministry of Education, Chongqing University, Chongqing 400045, China; Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing 100084, China; School of Environment, Beijing Key Laboratory for Emerging Organic Contaminants Control, State Key Joint Laboratory of Environment Simulation and Pollution Control (SKLESPC), Tsinghua University, Beijing 100084, China.
Abstract:
In this study, a convenient, sensitive, rapid and simple method was developed on microfluidic chip which was integrated with on-line complexing and laser-induced fluorescence detection. A rhodamine derivative (RD) was developed as a fluorescent chemosensor for Hg(II). It exhibited high selective recognition toward Hg(II) over other examined metal ions in water samples. Under the optimized conditions, the response was linearly proportional to the concentration of Hg(II) in the range of 0-70 μM with a detection limit of 0.031 μM. Satisfactory repeatability and reproducibility were achieved, with a relative standard deviation (RSD) of 6.62%. The established method was successfully applied for the determination of Hg(II) in environmental water samples (surface water, tap water, and waste water). Recoveries obtained for the determination of Hg(II) in spiking samples ranged from 85% to 103%.
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