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Updated: Feb 9, 2026

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
Published on: November 7, 2018
[Taq Man probe-based quadruple real-time PCR for detection of Salmonella paratyphi A/B/C and Salmonella typhi]
Longyan Xu1, Muyun Yuan1, Wei Sun1
1Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510623, China.
Objective:
Establishment and application of Taq Man probe-based quadruple real-time PCR for detection of Salmonella paratyphi A/B/C and Salmonella typhi. Primers specific to Salmonella paratyphi A( SPAP), Salmonella paratyphi B( SPBP), Salmonella paratyphi C( SPCP), and Salmonella typhi( STP) were designed.
Methods:
A method of Taq Man probe-based quadruple real-time PCR was established according to the distinction of the 5'end of the probe mark of TET, ROX, FAM and HEX. 5 strains of SPA, 4 strains of SPB, 7 strains of SPC and 11 strains of ST were identified by amplification from SPAP, SPBP, SPCP and STP.
Results:
While other serotypes of salmonella and17 strains of non-salmonella got negative results of amplification. Amplification rate of SPAP, SPBP, SPCP, and STP were 84. 5%, 101. 8%, 92. 4% and 90. 9%, respectively. The linear correlation coefficient( R~2) were 0. 996, 0. 975, 0. 996 and 0. 984, respectively.
Conclusion:
The PCR system is specific and sensitive for the identification of SPA, SPB, SPC and ST.
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