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Updated: Feb 9, 2026

VirWaTest, A Point-of-Use Method for the Detection of Viruses in Water Samples
Published on: May 11, 2019
[Studies and application method and quality control system for detecting hepatitis A virus in water for fruits and
Baohai Fang1, Zhiqin Yue1, Yuran Zhao1
1Inspection and Quarantine Technical Center of Shandong Exit& Entry Inspection and Quarantine Bureau, Qingdao 266002, China.
Objective:
To explore detecting methods and quality control standard system for hepatitis A virus( HAV) in water for fruits and vegetables production.
Methods:
100 μL of coliphage MS2( 2. 5 × 10~9 pfu / mL) was added into each water sample, and positively charged membrane filter method was used to capture virus. The virus extraction efficiency of each sample can be calculated according to standard curve of coliphage. Then the quality control system and real-time RT-PCR method were established.
Results:
This research compared the extraction efficiency of HAV and coliphage MS2 which were added into the water samples at the same time. The extraction efficiency of HAV was from 1. 24% to 32. 68%, and coliphage MS2 1. 64% to 14. 24%. The efficiency met the requirements of ISO / TS 15216-2-2013. Meanwhile, the HAV in 30 water samples were detected, and one was positive. The extraction efficiency of coliphage MS2 was from1. 24% to 24. 19%, and the standard deviation was 0. 0612.
Conclusion:
This research establish a quality control system to ensure the quality of test results.
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