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[Apple polyphenol inhibited the proliferation and migration of breast cancer cell MDA-MB-231 mediated by UHRF1]

Chunli Song1, Huanhuan Yang1, Xiaochao Song1

  • 1Department of Nutrition and Food Hygiene, School of Public Health, Medical College of Soochow University, Suzhou 215123, China.

Wei Sheng Yan Jiu = Journal of Hygiene Research
|June 16, 2018
PubMed
Abstract

Insights

Apple polyphenol effectively inhibits breast cancer cell growth and migration. It reduces key proteins like UHRF1 and MMP2, impacting cancer cell vitality and proliferation.

Area of Science:

  • Oncology
  • Molecular Biology
  • Phytochemistry

Background:

  • Breast cancer remains a significant global health challenge.
  • Identifying novel therapeutic agents from natural sources is crucial for cancer treatment.

Purpose of the Study:

  • To investigate the anti-cancer effects of apple polyphenol on the MDA-MB-231 breast cancer cell line.
  • To explore the underlying molecular mechanisms of apple polyphenol's action.

Main Methods:

  • MDA-MB-231 cells were treated with varying concentrations of apple polyphenol.
  • Cell vitality, proliferation, and migration were assessed using Trypan blue staining, CCK8 assay, and scratch assays.
  • Protein expression levels of UHRF1, MMP2, DNMT3a, and DNMT3b were analyzed via Western blot.

Main Results:

  • Apple polyphenol demonstrated dose-dependent inhibition of breast cancer cell vitality and proliferation.
  • Treatment with apple polyphenol significantly decreased the expression of UHRF1, MMP2, DNMT3a, and DNMT3b.
  • Inhibition of cell migration was more pronounced at higher concentrations (400-800 μg/mL).

Conclusions:

  • Apple polyphenol exhibits potent anti-proliferative and anti-migratory effects on MDA-MB-231 breast cancer cells.
  • The mechanism involves the downregulation of UHRF1, MMP2, and their downstream targets DNMT3a and DNMT3b.
  • These findings suggest apple polyphenol as a potential therapeutic candidate for breast cancer treatment.
Keywords:
DNMT3aDNMT3bapple polyphenolbreast cancer cell line MDA-MB-231cell proliferationmatrix metalloproteinases-2migrationubiquitinlike with PHD and ring finger domain 1

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