[Effects of fluoride on autophagy level in human neuroblastoma SH-SY5Y cells]
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Updated: Feb 9, 2026

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line
Published on: February 17, 2016
Sha Tang1, Shun Zhang1, Wei Chen1
1Department of Occupational and Environmental Health, School of Public Health, MOE Key Lab of Environment and Health, Tongji Medical College, Huazhong University of Science and Technology Wuhan 430030, China.
Objective:
To explore the effects of fluoride on autophagy level in human neuroblastoma SH-SY5Y cells.
Methods:
SH-SY5Y cells were treated with different concentrations( 20, 40, 60 mg/L) of fluoride for 24 h. Transmission electron microscope( TEM) was used to detect the ultrastructure of autophagosomes in SH-SY5Y cells. Acridine orange staining was used to examine the form of autophagic vesicles and Western blotting was used to check the expression levels of autophagy relevant proteins of Atg5, LC3 and P62 in SH-SY5 Y cells.
Results:
The TEM results showed the presence of autophagic ultrastructure in SH-SY5Y cells and fluoride could decrease the amount of autophagosomes. Acridine orange staining results showed that the amount of autophagic vesicles were obviously decreased in 40 and 60 mg/L Na F-treated groups compared with control, and 60 mg/L Na F-treated group was decreased the most. The expression level of Atg5 and LC3-II protein was dose-dependently decrease and P62 protein was dosedependently increased compared with control( P < 0. 05).
Conclusion:
Fluoride maysuppress the level of autophagy in SH-SY5Y cells.
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