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[Lycopene's protective effect on oxidative damage of L02 cells and its mechanism]
Qian Wang1, Xiaohong Wang1, Jingjing An1
1Department of Nutrition, Second Sanatorium of Qingdao Navy, Qingdao 266001, China.
Objective:
To explore lycopene 's protective effect on H_2O_2 induced oxidative damage of L02 cells and its mechanism.
Methods:
L02 cells were cultured by H_2O_2 to build the model of cellular oxidative damage. Different dose of lycopene was used to pretreat the cells, and cell survival rate was detected to verify the appropriate concentration. Then cellular ROS level, activity of cellular SOD and GSH-Px, cellular MDA content, and activity of ALT, AST and LDH in the culture medium were detected to observe lycopene 's effect on cellular oxidant damage. Finally, to observe lycopene 's activating effect on nuclear-translocation of Nrf2, L02 cells' nuclear protein was extracted to detect Nrf2 protein content, and also, mRNA expression level of Nrf2 target genes HO-1 and NQO1 was assayed to verify this mechanism.
Results:
Pretreatment of 10 μmol/Llycopene raised cellular viability of L02 cells on H_2O_2 culturing condition, reduced cellular ROS, enhanced enzymatic activity of cellular SOD and GSH-Px, reduced cellular MDA content, and depressed the activity of ALT, AST and LDH in culture medium. Lycopene also increased nuclear Nrf2 protein content and enhanced the expression of its target genes HO-1 and NQO1.
Conclusion:
Lycopene could protect L02 cells from H_2O_2 induced oxidative damage, probably by promoting nuclear-translocation of Nrf2 and activating expression of its target antioxidant genes.