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[Construction of LAMP detection method for poppy codeine in seasoning powders]
Liu Yang1, Yufei Chen1, Lei Wang1
1Department of Food Engineering, Jilin Business and Technology College, Changchun 130507, China.
Objective:
To establish the detection method for poppy codeine in seasoning power based on the loop-mediated isothermal amplification of DNA( LAMP).
Methods:
Establishment of the LAMP reaction system and detection of 8 commercially seasoning powders by the specificity test, optimum temperature test and sensitivity test of DNA samples of 12 poppies seeds, 11 spices leaves or seeds and 1 corn poppy seeds based on the specific genes sequence of codeine.
Results:
The DNA samples of the 12 poppies and the corn poppy showed the specific reaction, the optimum temperature was 66 ℃ and the minimum detectable concentration was 9 pg/μL, and the sensitivity was 100 times larger than that of PCR. There was codeine in one of the 8 commercially seasoning powders.
Conclusion:
The LAMP method applies to detect the poppy in seasoning powders with its high sensitivity, strong specificity, easy operation.
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