Shuttle vector for Escherichia coli, Pseudomonas putida, and Pseudomonas aeruginosa

A hybrid plasmid capable of replication in 2 different genera, Escherichia and Pseudomonas, was constructed. This plasmid DNA can be used as a cloning vector in E. coli and pseudomonades cells. The described hybrid plasmid pLD411 has been constructed on the basis of 2 small E. coli vector R-plasmids used in our laboratory and cryptic plasmid pWW2 or P. putida MT1. Plasmid pLD411 DNA was mapped with restrictases; its biological activity in transformations of different bacterial strains was studied, and the characteristics of transformed cells were also described.