Spectroscopy data of ceftriaxone-lysozyme interaction and computational studies.

Area of Science:

• Biochemistry
• Biophysical Chemistry
• Pharmacology

Background:

• Lysozyme is a crucial enzyme in biological systems.
• Ceftriaxone is a widely used antibiotic.
• Understanding drug-protein interactions is vital for drug development.

Purpose of the Study:

• To present spectroscopic and computational data on the lysozyme-ceftriaxone interaction.
• To investigate the influence of pH on this interaction.
• To complement a related research article on the same topic.

Main Methods:

• Fluorescence spectroscopy was employed to monitor the interaction.
• Synchrotron radiation circular dichroism (SRCD) spectroscopy provided insights into structural changes.
• Computational calculations were performed to support experimental findings.

Main Results:

• Spectroscopic data revealed changes in lysozyme upon ceftriaxone binding.
• The interaction was found to be pH-dependent, with distinct behaviors at neutral and acidic conditions.
• Computational results corroborated the experimental observations.

Conclusions:

• The study provides comprehensive data on lysozyme-ceftriaxone binding.
• pH significantly modulates the interaction, impacting binding affinity and mechanisms.
• This data serves as a valuable resource for further research in pharmaceutical sciences.