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Atrial natriuretic peptides stimulate renal gluconeogenesis
Biochemical and Biophysical Research Communications
|June 28, 1985
Summary
Atrial natriuretic peptide (ANP) and atrial extract (ANS) stimulate renal gluconeogenesis in rats. These effects require extracellular calcium and appear to involve the cAMP/cGMP system, suggesting basolateral action in proximal tubule cells.
Area of Science:
- Nephrology
- Endocrinology
- Molecular Physiology
Background:
- Renal gluconeogenesis is a vital metabolic process regulated by hormones.
- Atrial natriuretic peptide (ANP) is a hormone involved in cardiovascular and renal functions.
- The precise mechanisms by which ANP influences renal gluconeogenesis are not fully elucidated.
Purpose of the Study:
- To investigate the effect of ANP and atrial extract (ANS) on rat renal gluconeogenesis.
- To determine the signaling pathways and cellular localization involved in ANP-stimulated gluconeogenesis.
Main Methods:
- Rat renal cortical tubule suspensions were used to measure gluconeogenesis.
- Dose-response studies were performed with ANP and ANS.
- The roles of cAMP, cGMP, methoxamine, and extracellular calcium were examined.
- Experiments with isolated cells lacking membrane polarity were conducted.
Main Results:
- ANP and ANS dose-dependently stimulated renal gluconeogenesis from specific substrates.
- The effects of ANP and ANS were potentiated by cAMP and cGMP.
- Extracellular calcium concentrations above 1 mM were essential for the response.
- ANP and ANS did not affect isolated cells lacking membrane polarity.
Conclusions:
- ANP and ANS promote renal gluconeogenesis primarily through the cAMP and/or cGMP signaling systems.
- These peptides likely act at the basolateral membrane of proximal tubule cells.
- Extracellular calcium is critical for mediating the gluconeogenic effects of ANP and ANS.