Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mobility in the mitochondrial electron transport chain.

J Hochman, S Ferguson-Miller, M Schindler

    Biochemistry
    |May 7, 1985
    PubMed
    Summary

    Lateral diffusion of lipids and proteins in mitochondrial membranes is crucial for electron transport. Digitonin treatment alters lipid mobility and electron transfer rates, suggesting a dynamic aggregate model for mitochondrial components.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A Material Approach to Endangered Species Conservation: Characterization and 3D Imaging of Ballistic Damage in the Casques of Helmeted Hornbill (<i>Rhinoplax vigil</i>).

    Integrative organismal biology (Oxford, England)·2026
    Same author

    Central vein sign: A diagnostic biomarker in multiple sclerosis (CAVS-MS) study protocol for a prospective multicenter trial.

    NeuroImage. Clinical·2021
    Same author

    Fish early life stage toxicity prediction from acute daphnid toxicity and quantum chemistry.

    SAR and QSAR in environmental research·2021
    Same author

    Adiponectin stimulates glucose uptake in mouse blastocysts and embryonic carcinoma cells.

    Reproduction (Cambridge, England)·2020
    Same author

    [Multihospital use of imaging techniques in decentralized trauma care].

    Der Unfallchirurg·2019
    Same author

    Views and experiences with regard to antibiotic use of hospitalized patients in five European countries: a qualitative descriptive study.

    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2018

    Area of Science:

    • Mitochondrial biochemistry
    • Membrane biophysics
    • Cellular respiration

    Background:

    • Mitochondrial electron transport relies on the mobility of its components within the inner mitochondrial membrane.
    • Understanding the dynamics of these components is key to deciphering the efficiency of cellular respiration.

    Purpose of the Study:

    • To investigate the role of lateral diffusion in mitochondrial electron transport.
    • To measure diffusion coefficients of lipids, cytochrome c, and cytochrome oxidase in giant mitoplasts.
    • To explore the impact of different preparation techniques on membrane properties.

    Main Methods:

    • Utilized fluorescence redistribution after photobleaching (FRAP) to measure diffusion coefficients.
    • Employed phospholipid analogues and labeled proteins (cytochrome c, cytochrome oxidase) for FRAP analysis.
    • Compared mitoplasts prepared by sonication versus digitonin treatment.

    Main Results:

    • Lipid diffusion coefficients varied based on preparation: sonication yielded monophasic recovery (6 x 10(-9) cm2/s), while digitonin resulted in biphasic recovery (5 x 10(-9) cm2/s and 1 x 10(-9) cm2/s).
    • Digitonin treatment decreased lipid mobility and increased electron transfer rates, similar to cholesterol effects.
    • Diffusion coefficients for cytochrome c ranged from (3.5-7) x 10(-10) cm2/s, and for cytochrome oxidase, it was 1.5 x 10(-10) cm2/s.

    Conclusions:

    • Mitochondrial membrane components exhibit lateral diffusion, influencing electron transport.
    • Digitonin treatment alters membrane fluidity and electron transfer, indicating a dynamic interaction between lipids and proteins.
    • Results support a dynamic aggregate model where electron transport components exist in equilibrium between free diffusion and association.

    Related Experiment Videos