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A decreasing function describes a relationship where the output consistently declines as the input increases. This means that for any two input values, if one is greater than the other, the corresponding output is smaller. Mathematically, a function f is decreasing on an interval I if for every x1 < x2​ in I, f (x1) > f (x2). This type of behavior is visually identified on a graph that slopes downward from left to right.The nature of a function can be analyzed by calculating...
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Related Experiment Video

Updated: Feb 9, 2026

Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo
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Identification of Compounds That Decrease Glioblastoma Growth and Glucose Uptake in Vitro.

Catherine J Landis1, Sixue Zhang2, Gloria A Benavides3

  • 1Department of Cell, Developmental and Integrative Biology , University of Alabama at Birmingham , Birmingham , Alabama , United States.

ACS Chemical Biology
|June 16, 2018
PubMed
Summary
This summary is machine-generated.

Novel GLUT inhibitors were identified to target glioblastoma (GBM) tumor-initiating cells. These compounds reduce glucose uptake and GBM cell growth, offering a potential new therapeutic strategy for aggressive brain cancers.

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Area of Science:

  • Oncology
  • Cancer Metabolism
  • Neuro-oncology

Background:

  • Tumor heterogeneity in glioblastoma (GBM) hinders effective therapies.
  • Brain tumor-initiating cells (BTICs) drive GBM growth and exhibit metabolic plasticity.
  • GLUT3 is upregulated in BTICs, supporting survival in nutrient-poor environments.

Purpose of the Study:

  • To identify novel inhibitors targeting glucose transporter 3 (GLUT3) in GBM.
  • To evaluate the efficacy of identified inhibitors against GBM cells and BTICs.
  • To explore targeting metabolic reprogramming as a therapeutic strategy for GBM.

Main Methods:

  • Structure-based virtual screening (SBVS) was employed to identify potential GLUT inhibitors.
  • In vitro assays assessed the impact of compounds on GBM cell growth and glucose uptake.
  • Glycolytic stress tests were performed on patient-derived xenograft (PDX) cells.

Main Results:

  • Two novel compounds, SRI-37683 and SRI-37684, were identified through SBVS.
  • These compounds selectively inhibited GBM cell growth with minimal toxicity to normal cells.
  • Inhibition of glucose uptake, glycolytic capacity, and reserve capacity was observed in GBM PDX cells.

Conclusions:

  • Targeting GLUT3 offers a promising therapeutic avenue for glioblastoma.
  • Novel GLUT inhibitors demonstrate potential for treating GBM and other cancers.
  • SRI-37683 and SRI-37684 serve as valuable lead compounds for further drug development.