Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polyamine uptake by bovine adrenocortical cells.

J J Feige, E M Chambaz

    Biochimica Et Biophysica Acta
    |July 30, 1985
    PubMed
    Summary

    Bovine adrenocortical cells actively transport polyamines via an energy-dependent system. This specific uptake pathway may offer a rapid mechanism for regulating cellular polyamine levels, potentially influencing hormone action.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Inhibition of human placental endothelial cell proliferation and angiogenesis by netrin-4.

    Placenta·2015
    Same author

    Endocrine gland-derived endothelial growth factor (EG-VEGF) is a potential novel regulator of human parturition.

    Biology of reproduction·2014
    Same author

    Characterization of the adverse effects of nicotine on placental development: in vivo and in vitro studies.

    American journal of physiology. Endocrinology and metabolism·2013
    Same author

    Revisiting the role of hCG: new regulation of the angiogenic factor EG-VEGF and its receptors.

    Cellular and molecular life sciences : CMLS·2011
    Same author

    Insights into the role of genetic alterations in adrenocortical tumorigenesis.

    Molecular and cellular endocrinology·2008
    Same author

    Expression and localization of cellular prion and COMMD1 proteins in human placenta throughout pregnancy.

    Placenta·2007

    Area of Science:

    • Cell Biology
    • Endocrinology
    • Biochemistry

    Background:

    • Polyamines are crucial for cell growth and function.
    • Adrenocortical cells modulate polyamine levels via ornithine decarboxylase.
    • The role of polyamine transport in adrenocortical cells is not well understood.

    Purpose of the Study:

    • To investigate the presence and characteristics of polyamine uptake in bovine adrenocortical cells.
    • To determine if polyamine transport is linked to known amino acid transport systems.
    • To explore the potential implications of polyamine transport for rapid hormonal regulation.

    Main Methods:

    • Primary culture of bovine adrenocortical fasciculo-reticulata cells.
    • Measurement of polyamine (putrescine, spermine) uptake under various conditions (e.g., extracellular concentration, sodium dependence).
    • Cross-competition experiments and comparison with amino acid transport systems (A, L, N types).

    Main Results:

    • Bovine adrenocortical cells actively and energy-dependently accumulate extracellular polyamines.
    • Putrescine uptake involves both sodium-dependent, saturable, and sodium-independent components.
    • Spermine uptake is primarily sodium-independent, and cross-competition suggests shared transport mechanisms.
    • Polyamine uptake is distinct from known amino acid transport systems.

    Conclusions:

    • Bovine adrenocortical cells possess a specific, energy-dependent polyamine uptake system.
    • This transport system is independent of major amino acid transporters.
    • The findings suggest a potential rapid pathway for regulating intracellular polyamine levels, possibly involved in hormone action mechanisms.

    Related Experiment Videos