Related Experiment Video
Updated: Feb 8, 2026

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Published on: October 15, 2015
The relationship between volatile fatty acids accumulation and microbial community succession triggered by excess
Xiangkun Li1, Gaige Liu1, Shuli Liu1
1School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
Abstract:
The volatile fatty acids (VFAs) accumulation pattern and microbial community succession were studied during excess sludge (ES) alkaline fermentation at pH of 10.0 with expanded granular sludge blanket reactor over 5 cyclers. Microbial community shifted conspicuously as ES suffered alkaline fermentation. Both VFAs and acid-producing bacteria increased rapidly during the first 8 days fermentation time, and they showed a quite positive correlation relationship. In addition, soluble chemical oxygen demand (SCOD) also dramatically increased during the first 8 days, which implied 8 day was the optimum sludge retention time (SRT) for ES alkaline fermentation and VFAs accumulation time. Illumina Miseq Sequencing analysis indicated that Clostridium, Bacillus, Amphibacillus and Peptostreptococcaceae were the dominant bacteria genus to produce VFAs. Acetic acid took about 84% in total VFAs because among the total acid-producing bacteria most bacteria could produce acetic acid.
Related Concept Videos
Microbial Fermentation
Ecological Succession
Fermentation
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
Overview of Fatty Acid Metabolism
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
Gene Regulation in Microbial Communities: Quorum Sensing
Volatilization

