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Updated: Feb 8, 2026

Author Spotlight: Efficient Isolation of Primary Retinal Pigment Epithelial Cells from Adult Mice for Retinopathy Research
Published on: May 24, 2024
Retinal pigment epithelial cells as a therapeutic tool and target against retinopathies
Barbara Pavan1, Alessandro Dalpiaz2
1Department of Biomedical and Specialist Surgical Sciences, University of Ferrara, Ferrara, Italy.
Abstract:
Retinal pigment epithelium (RPE) is a cell monolayer essential for photoreceptor function and forming the blood-retinal barrier. RPE and retinal neurons share the same origin and a polarized cytoarchitecture. Several factors determine the phagocytosis and permeability of RPE, influencing photoreceptor renewal and drug delivery, efficacy and toxicity. Adult human RPE expresses neuronal markers in vitro, indicating a potential transdifferentiation. Degeneration of the RPE leads to death of photoreceptors and retinal neurons, resulting in the vision loss of retinopathy. Here, we suggest tools for cell engineering to discover new ways for activating the endogenous regeneration of barrier functions and/or of the retinal precursors in RPE cells.
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