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Clastogenecity evaluation of water of Lake Sevan (Armenia) using Tradescantia micronucleus assay
E A Aghajanyan1, R E Avalyan1, A E Simonyan1
1Laboratory of General and Molecular Genetics, RI "Biology", Faculty of Biology Yerevan State University, 8, Charents Str, 0025 Yerevan, Armenia.
Abstract:
The clastogenic effects of water samples in seven locations of Lake Sevan (Armenia) with the application of Trad-MCN (micronuclei) bioassay using Tradescantia (clone 02) were investigated. A significant increase in the frequency of micronuclei in tetrads of pollen microspores and tetrads with micronuclei exposed to the test samples compared to the control has been revealed. A multivariate analysis indicated linkage between the frequencies of occurrence of micronuclei in the cells and Ni and Co ions. The results were compared with the endpoints of another Tradescantia-based test system (stamen hair mutation test) performed on the same water samples and generation of the plant: occurrences of micronuclei in sporogenic cells coincided with that of non-surviving stamen hair.
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