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Fas activity mediates airway inflammation during mouse adenovirus type 1 respiratory infection

Laura J Adkins1, Caitlyn T Molloy1, Jason B Weinberg2

  • 1Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, MI, United States.

Virology
|June 17, 2018
PubMed

Insights

CD8 T cells help clear mouse adenovirus type 1 (MAV-1) and cause inflammation. Fas ligand (FasL) and Fas interactions partly mediate inflammation, but not viral clearance, by CD8 T cells.

Area of Science:

  • Immunology
  • Virology
  • Respiratory Medicine

Background:

  • CD8 T cells are crucial for controlling MAV-1 infection in the lungs.
  • CD8 T cell functions include viral clearance and inducing airway inflammation.
  • The role of Fas ligand (FasL)/Fas interactions in these CD8 T cell functions is unclear.

Purpose of the Study:

  • To investigate if FasL/Fas interactions mediate the antiviral and proinflammatory effects of CD8 T cells during MAV-1 infection.

Main Methods:

  • Compared MAV-1 infected C57BL/6 (B6) mice with Fas-deficient (lpr) mice.
  • Assessed viral replication, weight loss, pulmonary inflammation (histology), and cytokine levels.
  • Measured FasL and Fas expression in lung tissue.

Main Results:

  • Viral replication and weight loss were similar in B6 and lpr mice.
  • Pulmonary inflammation histology was similar, but MAV-1-infected lpr mice had lower lung mRNA and airway cytokine levels.
  • Fas deficiency did not affect virus-induced apoptosis in the lungs.

Conclusions:

  • FasL/Fas interactions contribute to CD8 T cell-mediated inflammation during MAV-1 infection.
  • These interactions are distinct from CD8 T cell antiviral functions.
  • CD8 T cell proinflammatory effects are partly mediated by Fas activation.
Keywords:
AdenovirusCD8 T cellsFasRespiratory infection

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