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Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification
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[Identification of Bombyx Batryticatus based on DNA barcoding technology].

Jing Jia, Lin-chun Shi, Hui Yao

    Yao Xue Xue Bao = Acta Pharmaceutica Sinica
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    This study introduces a two-dimensional DNA barcode system for identifying medicinal Bombyx Batryticatus. The system accurately distinguishes between Bombyx mori and Beauveria bassiana, crucial for Chinese medicine standardization.

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    Area of Science:

    • Pharmacology and Traditional Chinese Medicine
    • Molecular Biology and Genomics
    • Bioinformatics and Computational Biology

    Background:

    • Bombyx Batryticatus is a key component in traditional Chinese medicine, but its accurate identification is challenging.
    • Ensuring the quality and authenticity of medicinal materials is vital for therapeutic efficacy and patient safety.
    • Existing identification methods may struggle with mixed animal and fungal samples.

    Purpose of the Study:

    • To develop and validate a novel identification system for commercial Bombyx Batryticatus using DNA barcoding.
    • To establish an "Internet Plus" platform for seamless DNA barcode information exchange.
    • To address challenges in distinguishing between Bombyx mori and Beauveria bassiana in medicinal samples.

    Main Methods:

    • Extraction of total genomic DNA from medicinal materials using animal and plant DNA kits.
    • PCR amplification and sequencing of Cytochrome c Oxidase subunit I (COI) and Internal Transcribed Spacer (ITS) regions.
    • Construction of a two-dimensional DNA barcode system integrated with an "Internet Plus" platform.
    • Phylogenetic analysis using Neighbor-Joining (NJ) trees based on ITS sequences.

    Main Results:

    • The animal genomic DNA kit successfully extracted DNA from both Bombyx mori and Beauveria bassiana simultaneously.
    • COI sequences confirmed the presence of Bombyx mori, while ITS sequences identified Beauveria bassiana and other fungi.
    • NJ tree analysis of ITS sequences demonstrated clear differentiation among fungal species.
    • The developed DNA barcode system accurately identified mixed animal and fungal materials.

    Conclusions:

    • COI and ITS regions serve as reliable DNA barcodes for the stable and accurate identification of Bombyx Batryticatus.
    • The "Internet Plus" two-dimensional DNA barcode system effectively solves species identification issues in mixed medicinal materials.
    • This innovative system holds significant potential for promoting standardization and normalization within the Chinese medicinal materials market.