Related Experiment Video
Updated: Feb 8, 2026
06:54
Ex Vivo Perfusion of the Rodent Placenta
Published on: May 30, 2019
10.5K
Gestational changes in PRMT1 expression of murine placentas.
Anna Sato1, Jun-Dal Kim2, Hayase Mizukami3
1Department of Obstetrics and Gynecology, Juntendo University Faculty of Medicine, 2-1-1 Hongo, Bunkyoku, Tokyo 113-8421, Japan.
Placenta
|June 18, 2018
Summary
Protein arginine methyltransferase 1 (PRMT1) expression and protein arginine methylation levels change during mouse placental development. PRMT1 is highest in mid-gestation and decreases in late gestation, with reduced methylation in later stages.
Area of Science:
- Reproductive biology
- Developmental biology
- Biochemistry
Background:
- Placental development is crucial for fetal growth in mammals.
- Protein post-translational modifications regulate placental formation.
- The role of protein arginine methylation in placental development is largely unknown.
Purpose of the Study:
- To characterize the developmental expression of protein arginine methyltransferase 1 (PRMT1) in mouse placentas.
- To investigate the changes in protein arginine methylation status during placental development.
Main Methods:
- Quantitative PCR and Western blot to assess PRMT1 mRNA and protein levels.
- Immunohistochemistry and immunofluorescence to determine PRMT1 localization.
- Liquid chromatography-tandem mass spectrometry (LC-MS/MS) to quantify methylarginine levels.
Main Results:
- PRMT1 mRNA and protein expression peaked during mid-gestation and decreased in late gestation.
- PRMT1 was detected in various trophoblast cell subtypes, with specific localization in the labyrinth zone at embryonic day 13.
- Total methylarginine levels in placental proteins were significantly lower in late gestation compared to mid-gestation.
Conclusions:
- Significant developmental changes in PRMT1 expression and protein arginine methylation occur during mouse pregnancy.
- These findings offer foundational insights into PRMT1-mediated arginine methylation during placental development.
Keywords:
Arginine methylationAsymmetric dimethylarginine (ADMA)Methylarginines: monomethylarginine (MMA)PlacentaProtein arginine methyltransferase 1 (PRMT1)Splicing variantsSymmetric dimethylarginine (SDMA)More Related Videos
09:04Comprehensive Evaluation of the Effectiveness and Safety of Placenta-Targeted Drug Delivery Using Three Complementary Methods
Published on: September 10, 2018
10.1K
09:52Accurate and Simple Evaluation of Vascular Anastomoses in Monochorionic Placenta using Colored Dye
Published on: September 5, 2011
27.7K
Related Concept Videos
01:22Diabetes Mellitus: Type 2 and Gestational
5.0K
Type 2 diabetes, characterized by insulin resistance, arises when the insulin receptors on cells lose responsiveness to insulin, diminishing the cell's capacity to take up glucose, resulting in elevated blood glucose levels. To receive a diagnosis of Type 2 diabetes, a series of blood glucose tests are necessary to assess whether the blood glucose falls within normal parameters. If the result is out of the normal range, a patient may be diagnosed as prediabetic or diabetic, depending on the...
5.0K
01:42What is Gene Expression?
196.9K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
196.9K
01:36What is Gene Expression?
11.5K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
11.5K
02:48Expressing Solution Concentration
68.4K
A solute is a component of a solution that is typically present at a much lower concentration than the solvent. Solute concentrations are often described with qualitative terms such as dilute (of relatively low concentration) and concentrated (of relatively high concentration).
Concentrations may be quantitatively assessed using a wide variety of measurement units, each convenient for particular applications. Molarity (M) is a useful concentration unit for many applications in chemistry.
Concentrations may be quantitatively assessed using a wide variety of measurement units, each convenient for particular applications. Molarity (M) is a useful concentration unit for many applications in chemistry.
68.4K
01:58Cell Specific Gene Expression
16.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
01:26Algebraic Expressions
376
Algebraic expressions are essential in mathematics. They represent relationships through variables, constants, and operations. These expressions help describe patterns and solve problems in various mathematical fields. Understanding their components, classifications, and operations allows for efficient simplification and manipulation.Each algebraic expression consists of individual parts, including numbers and symbols, that work together to form meaningful mathematical statements. The numerical...
376