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Updated: Feb 8, 2026
11:10
Atomic Layer Deposition of Vanadium Dioxide and a Temperature-dependent Optical Model
Published on: May 23, 2018
12.5K
Marine Vanadium-Dependent Haloperoxidases, Their Isolation, Characterization, and Application
Ron Wever1, Bea E Krenn2, Rokus Renirie1
1University of Amsterdam, Van't Hoff Institute for Molecular Sciences, Amsterdam, The Netherlands.
Methods in Enzymology
|June 19, 2018
Summary
Vanadium-dependent haloperoxidases from various organisms are crucial for producing halogenated compounds that impact atmospheric chemistry and ozone depletion. This review details their sources, properties, and applications in biosynthesis.
Area of Science:
- Biochemistry
- Environmental Science
- Marine Biology
Background:
- Vanadium-dependent haloperoxidases (VHPs) are enzymes found in diverse organisms like seaweeds, cyanobacteria, fungi, and phytoplankton.
- These enzymes catalyze the formation of halogenated volatile compounds (halocarbons).
- Halocarbons released into the environment significantly influence atmospheric chemistry, contributing to ozone depletion.
Purpose of the Study:
- To provide a comprehensive survey of VHPs, covering their sources, isolation, and purification.
- To describe methods for detecting and quantifying VHP activity and their kinetic properties.
- To review recent advancements in VHP structure-function relationships and their biosynthetic applications.
Main Methods:
- Literature review of VHP sources, properties, and isolation techniques.
- Description of assays for VHP activity detection and quantification.
- Analysis of kinetic properties and structural data of VHPs.
Main Results:
- VHPs are widespread and play a key role in environmental halocarbon production.
- Detailed insights into enzyme isolation, purification challenges, and activity assays are provided.
- Recent structural and functional research highlights VHP versatility in biosynthesis.
Conclusions:
- Vanadium-dependent haloperoxidases are significant contributors to environmental halocarbon release.
- Understanding VHP properties and applications is crucial for studying atmospheric chemistry and developing biotechnological tools.
- This review offers practical guidance and highlights future research directions for VHPs.
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