Expression of an 8R-Lipoxygenase From the Coral Plexaura homomalla
Nathaniel C Gilbert1, David B Neau2, Marcia E Newcomer1
1Louisiana State University, Baton Rouge, LA, United States.
Abstract:
Methods are presented for the use of the coral 8R-lipoxygenase from the Caribbean sea whip coral Plexaura homomalla as a model enzyme for structural studies of animal lipoxygenases. The 8R-lipoxygenase is remarkably stable and can be stored at 4°C for 3 months with virtually no loss of activity. In addition, an engineered "pseudo wild-type" enzyme is soluble in the absence of detergents, which helps facilitate the preparation of enzyme:substrate complexes.
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