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Protective Effects of Shenfu Injection against Myocardial Ischemia-Reperfusion Injury via Activation of eNOS in Rats

Yan-Yan Wang1,2,3, Yan-Yan Li3, Lin Li1,2,3

  • 1Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine.

Biological & Pharmaceutical Bulletin
|June 19, 2018
PubMed

Insights

Shenfu injection (SFI) protects the heart from ischemia-reperfusion injury by activating the Akt/eNOS pathway. This traditional Chinese medicine reduces cardiac damage and improves heart function in rat models.

Area of Science:

  • Cardiovascular Pharmacology
  • Traditional Chinese Medicine
  • Molecular Cardiology

Background:

  • Myocardial ischemia-reperfusion injury (MIRI) is a major cause of heart damage.
  • Effective therapeutic strategies for MIRI are crucial.
  • Shenfu injection (SFI) is a traditional Chinese medicine with potential cardioprotective properties.

Purpose of the Study:

  • To investigate the protective effects of Shenfu injection (SFI) against myocardial ischemia-reperfusion injury (MIRI) in a rat model.
  • To elucidate the underlying mechanism of SFI's cardioprotective action, focusing on the Akt/eNOS signaling pathway.

Main Methods:

  • MIRI was induced in Sprague-Dawley rats by ligating the left anterior descending coronary artery followed by reperfusion.
  • Rats were pretreated with SFI or NG-nitro-L-arginine methyl ester (L-NAME).
  • Cardiac function, infarct size, serum biomarkers (CK, LDH, TNNI3), nitric oxide (NO) levels, and protein expression (p-Akt/Akt, p-eNOS/eNOS) were assessed.

Main Results:

  • SFI significantly reduced serum levels of cardiac injury biomarkers (CK, LDH, TNNI3).
  • SFI improved left ventricular function, including increased systolic and diastolic pressures, and enhanced ejection fraction and stroke volume.
  • SFI decreased myocardial infarct size and activated the Akt/eNOS signaling pathway, leading to increased NO production.

Conclusions:

  • Shenfu injection (SFI) demonstrates significant protective effects against myocardial ischemia-reperfusion injury in rats.
  • The cardioprotective mechanism of SFI involves the activation of the Akt/eNOS signaling pathway and subsequent increase in nitric oxide production.
  • SFI represents a promising therapeutic agent for managing MIRI.
Keywords:
NG-nitro-L-arginine methyl ester (L-NAME)Shenfu injectionischemia reperfusion injuryleft ventricular functionnitric oxide

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